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Updated: Jun 16, 2025
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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
Published on: October 18, 2019
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Novel competitive annealing-mediated isothermal amplification (CAMP)-based detection of epsilon toxin gene in
A Arun Prince Milton1, Torik Basar1, K Srinivas1
1Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, Meghalaya, India.
Anaerobe
|March 28, 2025
Abstract:
In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the etx gene, which encodes the epsilon toxin of Clostridium perfringens. The assay, performed at 60 °C for 60 min, demonstrated 100 % specificity and was 100 times more sensitive than endpoint PCR.
