Caffeic Acid Phenethyl Ester Inhibits Metastatic Properties of Acid-adapted Gastric Cancer Cells
- Sung-Chul Lim 1, Tae-Bum Lee 2, Song Iy Han 3
- Sung-Chul Lim 1, Tae-Bum Lee 2, Song Iy Han 3
- 1Department of Pathology, College of Medicine, Chosun University, Gwangju, Republic of Korea.
- 2Division of Premedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea.
- 3Division of Premedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea sihan@chosun.ac.kr.
- 0Department of Pathology, College of Medicine, Chosun University, Gwangju, Republic of Korea.
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View abstract on PubMed
Summary
This summary is machine-generated.Caffeic acid phenethyl ester (CAPE) effectively inhibits invasion in gastric cancer cells adapted to acidic environments. CAPE targets the AKT/β-catenin pathway, showing promise for treating gastric cancer in acidic tumor microenvironments.
Area Of Science
- Oncology
- Cancer Biology
- Molecular Medicine
Background
- Acidic tumor microenvironments enhance gastric cancer invasiveness and therapeutic resistance.
- Acid adaptation of gastric cancer cells leads to increased invasive potential.
- Investigating natural compounds for therapeutic strategies against acid-adapted cancers is crucial.
Purpose Of The Study
- To investigate the long-term effects of acidic adaptation on gastric cancer cells.
- To evaluate the anticancer properties of caffeic acid (CA) and caffeic acid phenethyl ester (CAPE).
- To elucidate the molecular mechanisms underlying CAPE's anti-invasive effects.
Main Methods
- Establishment of an acid-adapted gastric cancer cell line (SNU601-6.7) at pH 6.7.
- Assessment of cell invasiveness and matrix metalloproteinase (MMP) expression via invasion assays and qPCR.
- Evaluation of CA and CAPE effects on cell viability, apoptosis, invasion, and β-catenin signaling.
Main Results
- Acid-adapted cells showed increased invasiveness and elevated MMP2, MMP7, and MMP9 expression.
- Both CA and CAPE reduced cell viability and invasion; CAPE demonstrated superior efficacy.
- CAPE induced apoptosis and inhibited the AKT/β-catenin pathway, decreasing β-catenin levels and AKT/GSK3β phosphorylation.
Conclusions
- CAPE is a potent inhibitor of invasion in acid-adapted gastric cancer cells.
- The AKT/β-catenin pathway is a key target for CAPE's anti-invasive action.
- CAPE holds potential as a therapeutic agent for gastric cancer in acidic tumor microenvironments.
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