Related Experiment Video
Updated: May 11, 2026

MALDI Sample Preparation: the Ultra Thin Layer Method
Published on: April 29, 2007
DEAE-cellulose based ultrathin layer chromatography - mass spectrometry for protein separation and characterization
Anpu Wang1, Taghi Sahraeian1, Abraham K Badu-Tawiah1
1Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Ave, Columbus, OH, 43210, USA.
Background:
Ultrathin layer chromatography is an efficient method that is fast and requires a small amount of sample for the separation. This method may be valuable in for the separation of biological samples in many different industries such as pharmaceutical analyses as well as clinical analyses. This is the first example of the use of nanofibers of DEAE-cellulose for UTLC for protein separations. This is combined with the detection of the proteins using paper spray ionization mass spectrometry.
Results:
Protein and protein-complexes were readily separated and detected using the combination of these new separation and detection methods.
Significance:
This work illustrates the capability of DEAE-cellulose UTLC plates in protein separation and the feasibility of combining nanofiber UTLC with paper spray MS for further characterization. Successful characterization of both monomeric and complex proteins was obtained using this MS ionization methodology where charge state distributions of proteins showed effective performance of our UTLC separation procedure.
More Related Videos
12:34Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
05:45Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
Published on: June 8, 2021
Related Concept Videos
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
MALDI-TOF Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...