Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic diversity of pangolin coronaviruses reveals a key immuno-evasive substitution at spike residue 519.

Journal of virology·2026
Same author

Structural basis of Wnt signalosome extracellular complex assembly.

Cell·2026
Same author

A deep mutational scanning-informed protein language model predicts SARS-CoV-2 evolution dynamics with spatiotemporal resolution.

Nature microbiology·2026
Same author

Case Report: Transthoracic echocardiographic diagnosis of agenesis of mitral chordae tendineae with papillary muscle fusion.

Frontiers in cardiovascular medicine·2026
Same author

Association between right ventricular strain and subclinical left ventricular dysfunction in obstructive sleep apnea-hypopnea syndrome.

Sleep medicine·2026
Same author

A flexible semiparametric approach for robust causal inference with invalid instruments and unmeasured confounder.

Statistical methods in medical research·2026

Related Experiment Video

Updated: May 12, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

Deciphering SARS-CoV-2 evolution under antibody immune pressure.

Fanchong Jian1, Yunlong Cao1

  • 1Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, China; Changping Laboratory, Beijing, China.

Trends in Immunology
|March 28, 2025
PubMed
Summary

The SARS-CoV-2 virus evolves, challenging vaccines and antibodies. A new computational method predicts variant evolution using genomic data and deep mutational scanning, aiding future vaccine design.

More Related Videos

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay
05:49

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Published on: November 21, 2023

Application of Ha-CoV-2 Pseudovirus for Rapid Quantification of SARS-CoV-2 Variants and Neutralizing Antibodies
06:08

Application of Ha-CoV-2 Pseudovirus for Rapid Quantification of SARS-CoV-2 Variants and Neutralizing Antibodies

Published on: September 8, 2023

Related Experiment Videos

Last Updated: May 12, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay
05:49

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Published on: November 21, 2023

Application of Ha-CoV-2 Pseudovirus for Rapid Quantification of SARS-CoV-2 Variants and Neutralizing Antibodies
06:08

Application of Ha-CoV-2 Pseudovirus for Rapid Quantification of SARS-CoV-2 Variants and Neutralizing Antibodies

Published on: September 8, 2023

Area of Science:

  • Virology
  • Immunology
  • Computational Biology

Background:

  • The emergence of SARS-CoV-2 variants with immune escape mutations threatens the efficacy of current vaccines and neutralizing antibodies.
  • Understanding the evolutionary dynamics of SARS-CoV-2 is crucial for developing effective countermeasures.

Purpose of the Study:

  • To develop a computational framework for analyzing region-specific SARS-CoV-2 variant dynamics.
  • To predict the future evolutionary trends of SARS-CoV-2 variants in the context of population immunity.

Main Methods:

  • Utilized genomic surveillance data to track SARS-CoV-2 evolution.
  • Employed antibody deep mutational scanning (DMS) data to assess variant susceptibility to neutralization.
  • Integrated these data sources into a computational framework to model variant dynamics.

Main Results:

  • The framework successfully elucidated region-specific variant dynamics, highlighting key areas of viral evolution.
  • Predicted evolutionary trajectories of SARS-CoV-2 variants under varying levels of population immunity.
  • Identified potential future immune escape pathways.

Conclusions:

  • The developed computational framework provides a powerful tool for understanding and predicting SARS-CoV-2 evolution.
  • This approach can inform the design of next-generation vaccines and antibody therapies to combat emerging variants.
  • Genomic surveillance and DMS data are vital for proactive pandemic preparedness.