Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Methods of Medium Optimization01:28

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same author

Keeping Reviews Meaningful in the Era of AI.

Analytical chemistry·2026
Same author

Mass Spectrometry Imaging in ACS Journals.

ACS measurement science au·2026
Same author

Local Chemical Gradients in a Mammalian Cortex Measured In Vivo With a Silicon Nanodialysis Mass Spectrometry Platform.

Angewandte Chemie (International ed. in English)·2026
Same author

Profiling Endogenous Opioid Peptide Release from Adrenal Chromaffin Cells.

ACS chemical neuroscience·2026
Same author

<i>Precision Chemistry</i> and <i>Analytical Chemistry</i>Two Synergistic Journals.

Precision chemistry·2026

Related Experiment Video

Updated: May 11, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 27, 2013

Factorial-Design-Based Optimization of a Commercial MALDI-2 timsTOF Mass Spectrometer for Lipid Analysis.

Seth W Croslow1, Chen H Sirois2, Jonathan V Sweedler1,2

  • 1Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.

Journal of the American Society for Mass Spectrometry
|March 28, 2025
PubMed
Summary

Matrix-assisted laser desorption/ionization with laser postionization (MALDI-2 MS) is a key biomolecule analysis technique. Optimizing the timsTOF fleX system significantly boosts lipid signal intensity, enhancing lipidomics research.

More Related Videos

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
07:12

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Published on: August 23, 2024

Related Experiment Videos

Last Updated: May 11, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 27, 2013

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
07:12

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Published on: August 23, 2024

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Matrix-assisted laser desorption/ionization with laser postionization (MALDI-2 MS) is crucial for biomolecule analysis.
  • Commercial MALDI-2 systems, like the timsTOF fleX, are emerging, requiring optimization for specific applications.
  • Lipid analysis presents unique challenges due to the diverse nature of lipid species.

Purpose of the Study:

  • To comprehensively optimize the commercial timsTOF fleX system for enhanced lipid analysis using MALDI-2 MS.
  • To identify key instrumental parameters affecting lipid signal intensity and their interactions.
  • To maximize lipid signal detection for improved lipidomics studies.

Main Methods:

  • Utilized a factorial design of experiments (DOE) to systematically evaluate 13 instrumental parameters.
  • Performed over 1500 individual runs to assess parameter impacts on lipid signal intensity.
  • Optimized ion optics and MALDI-2 parameters in both positive and negative ion modes.

Main Results:

  • Identified laser shot frequency, collision RF, and pre-pulse storage as critical for ion transmission, yielding a ~5-fold signal increase.
  • Determined optimal TIMS In pressure and laser power for MALDI-2 performance.
  • Achieved signal enhancements of nearly two orders of magnitude for specific lipid species.

Conclusions:

  • Systematic optimization of the timsTOF fleX system significantly enhances lipid detection sensitivity via MALDI-2 MS.
  • The study provides crucial insights into parameter optimization for advanced lipidomics.
  • Optimized MALDI-2 MS protocols on commercial platforms enable more comprehensive lipid profiling.