Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction of "Integrated Electrochemical Oxidation and Biodegradation for Remediation of a Neonicotinoid Insecticide Pollutant".

ACS omega·2026
Same author

Retraction notice to "Advance research in biomedical applications on marine sulfated polysaccharide" [Int. J. Biol. Macromol. 194 (2022) 870-881].

International journal of biological macromolecules·2026
Same author

Association of blood biomarkers with p53 gene expression in male infertility.

Hormone molecular biology and clinical investigation·2026
Same author

Preclinical validation of tetrahydroquinoline derivatives as EGFR inhibitor inducing glioblastoma cell death.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026
Same author

Retraction notice to "Identification and characterization of major bioactive compounds from Andrographis paniculata (Burm. f.) extracts showed multi-biomedical applications" [Environ. Res. 242 (2024) 117763].

Environmental research·2026
Same author

Retraction notice to "Catalytic biodiesel production from Jatropha curcas oil: A comparative analysis of microchannel, fixed bed, and microwave reactor systems with recycled ZSM-5 catalyst" [Environ. Res. 258 (2024) 119474].

Environmental research·2026
Same journal

Influence of La<sub>2</sub>O<sub>3</sub> on the Structure, Thermal, and Luminescence Properties of Er<sup>3+</sup>-Doped TeO<sub>2</sub>-Ga<sub>2</sub>O<sub>3</sub>-BaF<sub>2</sub> Glass.

Luminescence : the journal of biological and chemical luminescence·2026
Same journal

The Comparative Study of Two New 6-Hydrazinylpurine Derivatives as "Turn-On" Fluorescence Chemosensors for the Detection of Zn<sup>2</sup>.

Luminescence : the journal of biological and chemical luminescence·2026
Same journal

Investigation of Zirconium-Lithium-Barium Oxides (ZrO<sub>2</sub>.Li<sub>2</sub>O.BaO) Nanocomposites' Optical, Fluorescence, and Thermophysical Characteristics for Optoelectronics and LEDs.

Luminescence : the journal of biological and chemical luminescence·2026
Same journal

Eco-Friendly Spectrofluorimetric Determination of Apixaban Using SDS-Enhanced Fluorescence in Pharmaceutical Forms and Plasma.

Luminescence : the journal of biological and chemical luminescence·2026
Same journal

Spectrofluorimetric Determination of Silodosin Using NBD-Cl Reagent: Applications to Capsules and Content Uniformity Testing.

Luminescence : the journal of biological and chemical luminescence·2026
Same journal

Low-Cost Lanthanum Nickel Manganese Oxide-Based Photodetector With PCBM and CuI Transport Layers for Eco-Friendly Applications.

Luminescence : the journal of biological and chemical luminescence·2026
See all related articles

Related Experiment Video

Updated: May 4, 2026

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
07:34

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

Published on: April 24, 2011

105.5K

Nuclear Staining and Fluorogenic Probe-Based Evaluation of ROS-Cytotoxic Potential Using AgNPs Synthesized From

Jayanthi Kamalanathan1, M S Suresh1, Venkatesh Rajendran2

  • 1Department of Chemistry, Government Arts College, Udhagamandalam, Nilgiris, Tamil Nadu, India.

Luminescence : the Journal of Biological and Chemical Luminescence
|March 29, 2025
PubMed
Summary
This summary is machine-generated.

This study identified phytochemicals in Cajanus trinervius (CT) leaf extract using GC-MS. The extract and its synthesized silver nanoparticles (CT-Ag NPs) showed significant apoptotic activity against cancer cells.

Keywords:
Cajanus trinerviusDAPIROScytotoxicityfluorescence

More Related Videos

Production and Detection of Reactive Oxygen Species ROS in Cancers
07:17

Production and Detection of Reactive Oxygen Species ROS in Cancers

Published on: November 21, 2011

69.8K
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
07:58

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

6.4K

Related Experiment Videos

Last Updated: May 4, 2026

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
07:34

Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death

Published on: April 24, 2011

105.5K
Production and Detection of Reactive Oxygen Species ROS in Cancers
07:17

Production and Detection of Reactive Oxygen Species ROS in Cancers

Published on: November 21, 2011

69.8K
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
07:58

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Published on: May 12, 2020

6.4K

Area of Science:

  • Phytochemistry
  • Nanotechnology
  • Biotechnology

Background:

  • Cajanus trinervius (CT) is a shrub with potential medicinal properties.
  • Phytochemical analysis is crucial for understanding plant-based therapeutic potential.
  • Silver nanoparticles (Ag NPs) synthesized from plant extracts offer novel applications.

Purpose of the Study:

  • To identify phytochemical compounds in Cajanus trinervius leaf extract.
  • To synthesize and analyze silver nanoparticles (CT-Ag NPs) using the extract.
  • To evaluate the cytotoxic and apoptotic effects of CT extract and CT-Ag NPs on A431 cell lines.

Main Methods:

  • Gas chromatography-mass spectrometry (GC-MS) for phytochemical profiling.
  • Microwave-assisted synthesis for CT-Ag NPs.
  • MTT assay, dual staining, DAPI staining, and DCFDA staining for cytotoxicity and apoptosis assessment.

Main Results:

  • GC-MS analysis revealed diverse phytochemicals including sesquiterpenoids, phenolic compounds, amino substances, and long-chain hydrocarbons.
  • Methanol, ethanol, and petroleum ether extracts contained 78, 82, and 38 chemical components, respectively.
  • Aqueous CT extracts and CT-Ag NPs demonstrated significant apoptotic activity and induced reactive oxygen species (ROS) in A431 cells.

Conclusions:

  • Cajanus trinervius leaf extract is rich in bioactive phytochemicals.
  • CT-Ag NPs synthesized via microwave irradiation are effective cytotoxic agents.
  • The study highlights the potential of CT extract and CT-Ag NPs in cancer therapy due to their apoptotic effects.