Amplified detection of β-glucuronidase activity based on cascade catalysis

Area of Science:

• Biochemistry
• Analytical Chemistry
• Enzyme Assays

Background:

• Beta-glucuronidase (β-GLU) is a key enzyme implicated in disease progression.
• β-GLU serves as a crucial biomarker and a potential therapeutic target.
• Sensitive and reliable detection methods for β-GLU activity are essential.

Purpose of the Study:

• To develop a novel cascade catalytic strategy for amplified detection of β-GLU activity.
• To establish a sensitive colorimetric method for quantifying β-GLU levels.
• To explore the utility of the developed system for inhibitor screening and disease treatment enhancement.

Main Methods:

• Utilized 8-hydroxyquinoline-β-D-glucuronide (8-HQG) as a substrate for β-GLU.
• Employed a cascade reaction involving β-GLU, Co²⁺, and ABTS for signal amplification.
• Developed a colorimetric assay for β-GLU detection based on H₂O₂-dependent oxidation.

Main Results:

• Achieved sensitive colorimetric detection of β-GLU activity.
• Established a limit of detection (LOD) of 0.022 U/mL for β-GLU.
• Demonstrated the effectiveness of the cascade catalytic system for β-GLU quantification.

Conclusions:

• The developed cascade catalytic strategy provides a sensitive and amplified method for β-GLU detection.
• This approach holds significant potential for applications in disease diagnosis and monitoring.
• The system is suitable for high-throughput screening of β-GLU inhibitors and guiding therapeutic strategies.