Effects of growth factor supplementation on the proliferation of cryopreserved canine amniotic membrane stem cells
- 1School of Animal Science and Food Engineering, Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, São Paulo, Brazil.
- 2Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of Sao Paulo, São Paulo, SP, Brazil.
- 3School of Animal Science and Food Engineering, Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, São Paulo, Brazil; Graduate Program in Translational Medicine, Drug Research and Development Center (NPDM), Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil.
- 4School of Animal Science and Food Engineering, Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, São Paulo, Brazil; University of Wyoming, School of Pharmacy, and the Biomedical Sciences Graduate Program, College of Health Sciences, Laramie, WY, 82072, USA.
- 0School of Animal Science and Food Engineering, Department of Veterinary Medicine, University of Sao Paulo, Pirassununga, São Paulo, Brazil.
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View abstract on PubMed
Summary
This summary is machine-generated.Growth factors like FGF-2, FGF-4, and PDGF-ββ enhance canine amniotic membrane-derived mesenchymal stem cell (AM-MSC) proliferation. This improves cell viability during long-term culture and after cryopreservation.
Area Of Science
- Stem Cell Biology
- Regenerative Medicine
- Veterinary Science
Background
- Amniotic membrane-derived mesenchymal stem cells (AM-MSCs) have great potential for proliferation and differentiation.
- Prolonged in vitro culture and cryopreservation can reduce the viability and expansion capacity of AM-MSCs.
- Canine AM-MSCs (cAM-MSCs) are a promising cell source for veterinary regenerative medicine.
Purpose Of The Study
- To investigate the impact of specific growth factors on the proliferation of advanced-passage cAM-MSCs.
- To assess the effects of growth factor supplementation before and after cryopreservation.
- To determine if growth factors influence the expression of key proliferation and apoptosis genes.
Main Methods
- Isolation and in vitro culture of cAM-MSCs from canine fetuses.
- Supplementation of cultures with fibroblast growth factor 2 (FGF-2), FGF-4, platelet-derived growth factor-ββ (PDGF-ββ), vascular endothelial growth factor-β (VEGF-β), and transforming growth factor-β1 (TGF-β1).
- Analysis of growth curves and quantification of SCAPER and TP53 gene expression via qPCR before and after cryopreservation.
Main Results
- Supplementation with FGF-2, FGF-4, and PDGF-ββ significantly increased cAM-MSC proliferation.
- Enhanced proliferation was observed both before and after cryopreservation.
- No significant alterations in the expression of SCAPER and TP53 genes were detected.
Conclusions
- FGF-2, FGF-4, and PDGF-ββ are effective in promoting cAM-MSC proliferation.
- Growth factor supplementation offers a potential strategy to improve cell viability in long-term cultures and enhance post-thaw recovery.
- These findings support the use of growth factors to optimize cAM-MSC expansion for therapeutic applications.
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