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Comprehensive strategies for constructing efficient CRISPR/Cas based cancer therapy: Target gene selection, sgRNA
Sathishbabu Paranthaman1, Chinnappa A Uthaiah2, Shadab Md3
1Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar 563103, Karnataka, India.
Advances in Colloid and Interface Science
|March 29, 2025
Summary
CRISPR/Cas gene editing offers advanced cancer therapy by precisely targeting mutations. This review details the CRISPR/Cas cancer therapy pipeline, addressing challenges like off-target effects for safer, more effective treatments.
Area of Science:
- Genomic Medicine
- Biotechnology
- Oncology
Background:
- Cancer arises from complex genetic and microenvironmental interactions.
- CRISPR/Cas systems enable precise genome editing for cancer research and therapy.
- Effective CRISPR/Cas cancer treatment requires optimizing gene targets and delivery methods.
Purpose of the Study:
- To outline the CRISPR/Cas cancer therapy pipeline.
- To identify strategies for optimizing guide RNAs (gRNAs and sgRNAs).
- To review delivery modalities and current clinical applications.
Main Methods:
- Literature review of CRISPR/Cas technology in cancer therapy.
- Analysis of gene targeting strategies and optimization of gRNAs/sgRNAs.
- Exploration of viral, non-viral, and extracellular vesicle delivery systems.
Main Results:
- CRISPR/Cas technology has significantly advanced cancer modeling and mutation identification.
- Key challenges include off-target effects, specificity, and immunogenicity.
- Various delivery methods are being explored to enhance therapeutic efficacy.
Conclusions:
- A comprehensive pipeline for CRISPR/Cas cancer therapy development is crucial.
- Optimizing gene targets and delivery systems improves precision, effectiveness, and safety.
- Ongoing research and clinical trials highlight the therapeutic potential of CRISPR/Cas in oncology.
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