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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Chitosan-stabilized copper oxide nanoparticles: A novel colorimetric approach for ascorbic acid sensing.
Nazish Kanwal1, Mansoor Khan1, Saeed Ahmad Khan2
1Department of Chemistry, Kohat University of Science and Technology, Kohat, 26000, KP Pakistan.
Analytical Biochemistry
|March 29, 2025
Summary
A novel nanozyme, cross-linked chitosan-stabilized copper oxide nanoparticles (CuO@C-CS), was developed for sensitive ascorbic acid detection. This biodegradable sensor offers a promising tool for analyzing vitamin C in food and biomedical samples.
Area of Science:
- * Nanomaterials Science
- * Analytical Chemistry
- * Biochemistry
Background:
- * Ascorbic acid (vitamin C) plays a crucial role in human health and is linked to various diseases.
- * Accurate and sensitive detection of ascorbic acid is vital for biomedical and food analysis.
- * Existing detection methods may lack efficiency, sensitivity, or biodegradability.
Purpose of the Study:
- * To synthesize and characterize novel cross-linked chitosan-stabilized copper oxide nanoparticles (CuO@C-CS).
- * To develop a sensitive and selective nanozyme-based sensor for ascorbic acid detection.
- * To evaluate the sensor's performance and applicability in real-world samples like orange juice.
Main Methods:
- * Synthesis of CuO@C-CS nanoparticles using a simple and efficient strategy.
- * Characterization of the synthesized material using FTIR, XRD, SEM, EDX, and TGA.
- * Development of a colorimetric sensing assay utilizing the peroxidase-mimicking activity of CuO@C-CS and TMB substrate.
Main Results:
- * Successful synthesis and characterization of biodegradable CuO@C-CS nanoparticles confirmed by various techniques.
- * The CuO@C-CS nanozyme demonstrated efficient peroxidase-mimicking activity for ascorbic acid detection.
- * The sensor exhibited excellent sensitivity (LOD: 0.24 μM), selectivity, and a wide linear range (1-96 μM).
- * The sensor was successfully applied for the qualitative and quantitative analysis of ascorbic acid in orange juice samples.
Conclusions:
- * Cross-linked chitosan-stabilized copper oxide nanoparticles (CuO@C-CS) offer a promising non-toxic and biodegradable platform for nanozyme development.
- * The developed sensor provides a highly sensitive, selective, and efficient method for ascorbic acid determination.
- * This approach has significant potential for application in food quality control and biomedical diagnostics.
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