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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jul 7, 2026

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
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Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Published on: December 6, 2014

Performance Evaluation of a Next-Generation Sequencing-Based T-Cell Receptor Gene Rearrangement Assay.

Danica Wiredja1, Diane Libert2, Diwash Jangam3

  • 1Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, Colorado.

The Journal of Molecular Diagnostics : JMD
|March 30, 2025
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) T-cell receptor (TCR) clonality testing aids in diagnosing T-cell malignancies. This study optimized interpretation criteria for NGS-based TCR assays, improving diagnostic yield and identifying analytical challenges.

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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

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14:14

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

Area of Science:

  • Immunology
  • Oncology
  • Genetics

Background:

  • T-cell receptor (TCR) gene rearrangement clonality studies are crucial for diagnosing T-cell malignancies.
  • Standardized interpretation criteria for next-generation sequencing (NGS) based TCR assays are lacking.
  • Atypical T-cell proliferations require accurate diagnostic methods.

Purpose of the Study:

  • To assess the performance of an in-house NGS-based TCR clonality assay.
  • To optimize interpretation criteria for improved diagnostic accuracy.
  • To identify recurrent analytical challenges in TCR clonality testing.

Main Methods:

  • Analysis of 619 cases using concurrent pathology reports as clinical truth.
  • Evaluation of the existing assay's performance (74% sensitivity, 85% specificity).
  • Application of receiver operating characteristic analysis to identify optimized interpretation criteria.

Main Results:

  • The current NGS-based TCR clonality assay demonstrated 74% sensitivity and 85% specificity.
  • Optimized interpretation criteria yielded only marginal improvements in diagnostic yield.
  • Discrepancies were primarily attributed to technical limitations or diagnostic nuances.

Conclusions:

  • This study provides an objective framework for establishing interpretation criteria for NGS-based TCR clonality tests.
  • The findings offer guidance for laboratories implementing similar diagnostic assays.
  • Further optimization is needed to address technical and biological complexities in TCR clonality assessment.