Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emission dipole orientation reveals dynamic single-molecule interactions with 2D crystals at solvent interfaces.

Nature communications·2026
Same author

All-water supercapacitor enabled by 1-nm clay channels.

Nature communications·2026
Same author

Fabrication of ultra-small bimorph cantilevers for high-speed AFM of biological samples.

Nanotechnology·2026
Same author

Nanopore Trap for Label-Free Fingerprinting of Surface-modified Single Nanoparticles.

Small methods·2025
Same author

Single-Photon Single-Particle Tracking.

bioRxiv : the preprint server for biology·2025
Same author

A Comprehensive Analysis of Combined AFM/SEM Systems for In-Situ Nanoscale Characterizations and Multiparametric Correlative Microscopy.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2025
Same journal

Reconfigurable Logic-in-Memory Oxide Transistors Enabled by Transferable Ferroelectric HZO.

ACS nano·2026
Same journal

Specific Multimodal Imaging of Deep-Seated Tumor with High Intratumoral Retention <i>via In Situ</i> Assembly of Probes.

ACS nano·2026
Same journal

Emergence of Nonuniform Strain-Induced Exciton Species in Bilayer Transition Metal Dichalcogenides.

ACS nano·2026
Same journal

Fiber-Optic Quantum Dots Sensor for Dynamic and Quantitative Thermal Monitoring of Spheroids toward Single-Cellular Resolution.

ACS nano·2026
Same journal

Nitric Oxide-Mediated Minimally Invasive Neuromodulation through Gut-Brain Axis via a Bioelectronic Microdevice for Relieving Depressive Symptoms.

ACS nano·2026
Same journal

Tailorable Topological Multimode Nanolaser with Mutually Incoherent Modes.

ACS nano·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Controlled Sensing of User-Defined Aptamer-Based Targets Using Scanning Ionic Conductance Spectroscopy.

Helena Miljkovic1,2, Lely Feletti1, Gordanna Pistoletti Blanchet3

  • 1Laboratory of Nanoscale Biology (LBEN), Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), 1015 Lausanne, Switzerland.

ACS Nano
|March 31, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new nanopore sensing system using programmable aptamers on DNA carriers for improved disease biomarker detection. The enhanced spatial and velocity control significantly boosts detection accuracy and signal-to-noise ratio for clinical applications.

Keywords:
SICSaptamerproteinsingle molecule detectionsolid-state nanopore

More Related Videos

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jun 8, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Solid-state nanopores are promising for early disease biomarker detection.
  • Current methods using DNA carriers and free translocation have low accuracy and signal-to-noise ratio (SNR) due to uncontrolled translocations.
  • These limitations hinder clinical application of nanopore technology.

Purpose of the Study:

  • To develop a nanopore-based system for sensing molecules in biological fluids with enhanced accuracy and control.
  • To overcome the limitations of standard nanopore approaches by enabling controlled translocation and repeated analyte scanning.
  • To improve detection rates and enable multiplexed sensing for disease biomarker discovery.

Main Methods:

  • A nanopore system utilizing programmable aptamer sequences attached to DNA carriers tethered to glass surfaces.
  • Employing scanning ion conductance spectroscopy (SICS) for controlled spatial and velocity translocation (x, y, z directions).
  • Designing DNA carriers with multiple aptamer binding sites for increased experimental yield and multiplexing.

Main Results:

  • Achieved a detection rate of up to 74%, a significant improvement over the 14% from standard methods.
  • Demonstrated repeated scanning of the same aptamer-target site over 5 times, enhancing data reliability.
  • Enabled increased densities of aptamer target sites on DNA carriers, paving the way for multiplexed sensing.

Conclusions:

  • The proposed SICS-based nanopore system offers superior control and significantly higher detection rates for disease biomarkers.
  • Programmable aptamer sequences and DNA carriers allow for user-defined sensing capabilities and potential expansion to various biomarkers.
  • This technology holds promise for advancing early diagnostic applications in clinical settings.