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Verification of an Algorithm for Detection of Unstable Haemoglobin Variants With Sysmex XN-10 Yielded a High Degree

Madeleen Bosma1, Richard M Noordervliet1, Mercedeh Tajdar2

  • 1Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Centre, Leiden, the Netherlands.

International Journal of Laboratory Hematology
|March 31, 2025
PubMed
Abstract

No abstract available in PubMed .

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...