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Lipid transfer between phosphatidylcholine vesicles and human erythrocytes: exponential decrease in rate with

J E Ferrell, K J Lee, W H Huestis

    Biochemistry
    |June 4, 1985
    PubMed
    Summary
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    Phospholipid transfer between vesicles and cells follows specific kinetics, influenced by lipid acyl chain length. Shorter chains transfer faster, suggesting aqueous phase monomer transfer is the primary mechanism.

    Area of Science:

    • Biochemistry
    • Membrane Biology
    • Physical Chemistry

    Background:

    • Phospholipid transfer between biological membranes is crucial for membrane homeostasis and function.
    • Understanding the mechanisms of this transfer is key to elucidating cellular processes.

    Purpose of the Study:

    • To investigate the kinetics of phospholipid transfer from vesicles to erythrocytes.
    • To determine the influence of membrane concentration and lipid acyl chain composition on transfer rates.

    Main Methods:

    • Studied phospholipid transfer rates from sonicated vesicles to human erythrocytes.
    • Varied membrane concentrations and lipid acyl chain compositions (homologous saturated phosphatidylcholines).
    • Analyzed kinetic behavior using models of transient contact and aqueous phase monomer transfer.

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    Main Results:

    • Phospholipid transfer exhibited saturable first-order kinetics concerning both cell and vesicle concentrations.
    • Transfer rates were profoundly affected by acyl chain composition, decreasing exponentially with increasing chain length for saturated phosphatidylcholines.
    • Rapid inter-red cell transfer of dilauroylphosphatidylcholine contradicted predictions of collisional transfer models.

    Conclusions:

    • Observed kinetics support phospholipid transfer via monomer passage through an aqueous phase.
    • The relationship between acyl chain length and transfer rate likely reflects the energetics of aqueous monomer transfer.
    • Findings provide insights into the biophysical mechanisms governing intermembrane lipid exchange.