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Jelleine-I Membrane Interaction-related Biological Properties and Antimicrobial Activity against MDR, XDR, and

Adrielle Pieve de Castro1, Julio Cesar Moreira Brito2, Wanderson Aparecido Brandão Candido3

  • 1Laboratório de Radioisótopos, Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Campus Pampulha, Belo Horizonte, Minas Gerais 31270-901, Brazil.

ACS Omega
|March 31, 2025
PubMed

Insights

Jelleine-I, an antimicrobial peptide from bee jelly, effectively combats carbapenem-resistant Acinetobacter baumannii. It disrupts bacterial membranes with low toxicity, showing promise as a novel antibacterial agent.

Area of Science:

  • Microbiology
  • Biochemistry
  • Biophysics

Background:

  • Emerging bacterial infections, particularly carbapenem-resistant Acinetobacter baumannii (CRAB), represent a significant global health threat.
  • Antimicrobial peptides (AMPs) are emerging as a promising alternative to conventional antibiotics due to their unique mechanisms of action.
  • Jelleine-I, an AMP derived from bee royal jelly, has shown broad-spectrum antibacterial activity.

Purpose of the Study:

  • To evaluate the efficacy of jelleine-I against clinical isolates of CRAB.
  • To investigate the interaction mechanism of jelleine-I with bacterial membranes.
  • To assess the safety profile of jelleine-I regarding hemolytic activity and cytotoxicity.

Main Methods:

  • Microbiological assays were conducted to determine the minimum inhibitory concentration (MIC) of jelleine-I against CRAB isolates.
  • Biophysical techniques were employed to study the interaction between jelleine-I and biomimetic bacterial membranes.
  • Hemolytic activity assays and cytotoxicity tests on VERO and HaCaT cells were performed.

Main Results:

  • Jelleine-I exhibited significant antibacterial activity against CRAB isolates, with MIC values ranging from 8-16 μM.
  • Biophysical studies indicated that jelleine-I disrupts the organization of phospholipid bilayers, suggesting membrane permeabilization as a key mechanism.
  • Jelleine-I demonstrated negligible hemolytic activity and low toxicity towards mammalian cells.

Conclusions:

  • Jelleine-I is a potent antimicrobial agent effective against multidrug-resistant Acinetobacter baumannii.
  • The primary antimicrobial action of jelleine-I involves the disruption of the bacterial membrane.
  • Jelleine-I represents a promising candidate for the development of novel therapeutics against challenging bacterial infections.

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