Transcripts from the src-1(cj293) mutant can encode a SRC-1 molecule lacking the SH2 domain in Caenorhabditis elegans

Previous studies suggest that the src-1 ( cj293 ) mutation is an activated src-1 allele in C. elegans with the potential to encode a molecule lacking the SH2 domain. src-1 ( cj293 ) is a deletion with breakpoints in introns 3 and 5, deleting exons 4 and 5, which encode the SH2 domain. If exon 3 is spliced to exon 6, the reading frame is maintained. Here, RNA seq of src-1 ( cj293 ) mutants showed that the exon 3 to exon 6 splice does not occur in src-1 (+) but is robustly present in src-1 ( cj293 ) . Thus, src-1 ( cj293 ) produces a transcript that can encode a SRC-1 molecular lacking the SH2 domain, which leads to overactive SRC-1 in growth cones of VD neurons during their outgrowth ( i.e. src-1 ( cj293 ) might be a constitutively-active mutation).