Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epithelial water transport in a balanced gradient system.

R T Mathias

    Biophysical Journal
    |June 1, 1985
    PubMed
    Summary

    Epithelial fluid transport is governed by active solute transport. Standing osmotic and hydrostatic gradients balance, uncoupling intercellular clefts from reabsorption rates for effective fluid regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Transgenic expression of AQP1 in the fiber cells of AQP0 knockout mouse: effects on lens transparency.

    Experimental eye research·2010
    Same author

    Functional characterization of a human aquaporin 0 mutation that leads to a congenital dominant lens cataract.

    Experimental eye research·2008
    Same author

    Local osmosis and isotonic transport.

    The Journal of membrane biology·2006
    Same author

    Transmural gradients in Na/K pump activity and [Na+]I in canine ventricle.

    Biophysical journal·2005
    Same author

    Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions.

    The Journal of physiology·2005
    Same author

    Gap junctional coupling in lenses from alpha(8) connexin knockout mice.

    The Journal of general physiology·2001

    Area of Science:

    • Physiology
    • Biophysics
    • Cell Biology

    Background:

    • Epithelial fluid transport is crucial for physiological homeostasis.
    • Understanding the interplay of osmotic and hydrostatic pressure gradients is key to explaining fluid movement across epithelia.

    Purpose of the Study:

    • To investigate the relationship between epithelial fluid transport, standing osmotic gradients, and standing hydrostatic pressure gradients.
    • To elucidate the mechanisms governing fluid reabsorption and flow within intercellular clefts.

    Main Methods:

    • Utilized a perturbation expansion of governing equations for epithelial transport.
    • Applied assumptions of small lateral intercellular space volume and high membrane osmotic permeability relative to solute permeability.

    Main Results:

    • Fluid reabsorption rate is determined by active solute transport across lateral membranes.
    • Longitudinal hydrostatic pressure gradients drive fluid flow in intercellular clefts.
    • Standing osmotic gradients counter transmembrane fluid movement, balancing longitudinal gradients.

    Conclusions:

    • Intercellular cleft gradients are uncoupled from reabsorption rates due to balanced longitudinal forces.
    • Water movement occurs across apical and lateral membranes.
    • Fluid flow through tight junctions depends on a parameter scaling solute flow resistance, suggesting leaky epithelia can behave as tight ones for fluid flow.

    Related Experiment Videos