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Updated: May 16, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Design and Preliminary Screen of Antisense Oligonucleotides
Nofar Mor1, Sharon Avkin-Nachum2, Dan Dominissini2,3
1Cancer Research Center and Wohl Institute for Translational Medicine, Ramat Gan, Israel. nofar.mor@sheba.health.gov.il.
Abstract:
Antisense Oligonucleotides (ASOs) are short, synthetic oligonucleotides that specifically bind to target RNA sequences to modulate their expression. ASOs have applications in both research and clinical therapy, with more than eight FDA-approved products and numerous others in clinical development. The functionality of ASOs is determined by their nucleotide sequence and the chemical modifications they carry. These modifications can define the ASO's function, and increase its stability, safety, and distribution in the body. In this chapter, we offer a comprehensive guide to designing RNAse-H1 recruiting ASOs, including guideline for sequence selection and chemical modifications. Additionally, we will outline a protocol for initial screenings of ASO libraries, providing recommendations for selection of the suitable screening platform.

