Exploring the Perturbation of Biological Processes Caused by Gene Upregulation Using Knock-In Transfection,
Isabella R Garcia1, Lee D Larcombe2
1Applied Exomics Ltd (Apexomic), Stevenage, Hertfordshire, UK.
Abstract:
Overexpressing a gene in a mammalian cell line can be an extremely powerful technique to investigate and explore a gene's biological functions and associated pathways. Here we describe how combining wet-lab methodology and in-silico transcriptomic approaches can aid in the discovery of unknown gene functions, perturbation of molecular processes or the elucidation of mechanism of action.
More Related Videos
09:44High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Published on: March 3, 2015
07:34The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
Published on: February 16, 2017
