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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

A Generalizable Screening Platform for Developing Functional Aptasensors.

Micaela Belleperche¹, Jiawen Liu¹, Yuhao Chen²

¹Department of Chemistry, Faculty of Science, McGill University, Montreal, QC H3A 0B8, Canada.

Analytical Chemistry

|March 31, 2025

Summary

Designing effective aptasensors for contaminants like ochratoxin A (OTA) is challenging. This study introduces a novel surface plasmon resonance (SPR) assay to rapidly identify optimal DNA probes, accelerating biosensor development.

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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Area of Science:

Biotechnology
Biosensor Development
Molecular Recognition

Background:

Aptamers are key components in aptasensors, enabling target detection through conformational changes.
Signal generation often relies on the displacement of complementary probes upon aptamer-target binding.
Efficient probe design is critical but challenging, typically requiring extensive screening.

Purpose of the Study:

To investigate factors influencing probe displacement efficiency in aptamer-based biosensors.
To develop a rapid and generalizable method for evaluating probe displacement.
To improve the design of aptasensors for contaminants like ochratoxin A (OTA).

Main Methods:

Exploration of probe properties (length, affinity, melting temperature) for OTA aptamer.
Development of a novel surface plasmon resonance (SPR) assay to measure target-induced probe displacement.
Correlation of SPR displacement data with fluorescence recovery from quencher-labeled probes.

Main Results:

Probe length, affinity, and melting temperature did not reliably predict displacement efficiency.
The developed SPR assay rapidly and accurately measured probe displacement.
SPR results correlated well with fluorescence-based measurements, validating the assay's effectiveness.

Conclusions:

A novel SPR-based assay enables rapid assessment of probe displacement efficiency.
This method accelerates the identification of optimal probes for aptasensor construction.
The approach is adaptable to various aptamers, facilitating broader aptasensor development.