A trinity sensing platform for uric acid detection in clinical samples using Fe-functionalized carbon quantum dot...

Area of Science:

• Biomarker detection
• Nanozymes
• Clinical diagnostics

Background:

• Early disease detection relies on accurate biomarker identification.
• Iron-functionalized carbon quantum dots (Fe-CQD) exhibit peroxidase-like activity.
• Uric acid detection is crucial for diagnosing various health conditions.

Purpose of the Study:

• To develop a simple, reliable, and visual sensing platform for uric acid detection.
• To utilize Fe-CQD nanozymes for enhanced biomarker detection.
• To integrate advanced imaging and spectroscopy for clinical diagnostics.

Main Methods:

• Synthesized Fe-CQD from dandelion biomass.
• Employed Fe-CQD nanozymes to catalyze H2O2 for ROS generation.
• Developed a visual sensing platform using absorption spectra, microplate reader, and smartphone imaging for uric acid detection.

Main Results:

• Achieved low detection limits for uric acid (0.31–0.83 μM).
• Demonstrated high accuracy and consistency with standard hospital methods in clinical blood samples.
• Showcased robust anti-interference capabilities and practicality for real-world applications.

Conclusions:

• The Fe-CQD nanozyme-based visual sensing platform offers a reliable tool for uric acid detection.
• This innovative platform highlights the potential of nanozymes in clinical diagnostics.
• The study lays the foundation for integrating intelligent, portable sensing technologies into healthcare.