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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: May 16, 2025

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

Statistical analysis of spatial patterns in tumor microenvironment images.

Mohamed M Benimam¹, Vannary Meas-Yedid¹, Suvadip Mukherjee^1,2

¹Institut Pasteur, Université de Paris Cité, CNRS UMR 3691, BioImage Analysis Unit, Paris, France.

Nature Communications

|March 31, 2025

Summary

Spatiopath is a new framework for analyzing immune cell patterns in tumors. It reveals significant spatial associations between immune cells and tumor regions, aiding in understanding immune responses and identifying biomarkers.

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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Related Experiment Videos

Last Updated: May 16, 2025

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Author Spotlight: Unlocking Insights into the Immune Cell Landscape of Tumors

Published on: August 18, 2023

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Area of Science:

Immunology
Computational Biology
Oncology

Background:

Automated cell identification and imaging allow visualization of immune cells in human tumors.
A framework for analyzing spatial patterns in the tumor microenvironment (TME) is needed.

Purpose of the Study:

Develop Spatiopath, a novel null-hypothesis framework to identify statistically significant immune cell associations within the TME.
Extend Ripley's K function to analyze cell-cell and cell-tumor spatial interactions.

Main Methods:

Utilize embedding functions to map cell contours and tumor regions.
Validate Spatiopath using synthetic simulations.
Apply the framework to multi-color images of lung tumor sections.

Main Results:

Spatiopath identified significant spatial patterns of immune cells within the tumor microenvironment.
Mast cells were observed accumulating near T cells and the tumor epithelium.
Distinct spatial organizations were revealed, with mast cells clustering near the epithelium and T cells positioned farther away.

Conclusions:

Spatiopath provides a robust framework for analyzing immune cell spatial distribution in tumors.
The findings enhance the understanding of immune responses within the tumor microenvironment.
This approach may aid in identifying prognostic biomarkers for patient outcomes.