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Related Concept Videos

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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
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SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
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An unbiased cell-culture selection yields DNA aptamers as novel senescent cell-specific reagents.

Keenan S Pearson1, Sarah K Jachim1, Caroline D Doherty2

  • 1Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Rochester, Minnesota 55905, United States.

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Researchers developed novel DNA aptamers to specifically identify senescent cells. This new method targets fibronectin and shows promise for detecting cellular senescence in aging tissues.

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Techniques to Induce and Quantify Cellular Senescence

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Cellular senescence is a key factor in aging and disease, characterized by irreversible cell-cycle arrest.
  • Current methods for detecting senescent cells rely on multiple biomarkers, lacking a universal, stand-alone indicator.
  • Identifying specific markers for senescent cells is crucial for understanding aging and age-related diseases.

Purpose of the Study:

  • To develop novel, highly specific DNA aptamers for identifying senescent cells.
  • To validate the specificity and utility of these aptamers in cellular and tissue models.
  • To explore new diagnostic tools for cellular senescence.

Main Methods:

  • Unbiased cell culture selections using vast libraries of random DNA aptamers.
  • Screening aptamers against senescent and non-senescent mouse fibroblasts.
  • Identifying molecular targets of selected aptamers using biochemical assays.
  • Validating aptamer performance in naturally aged mouse tissues and transgenic models.

Main Results:

  • Successfully identified senescent cell-specific DNA aptamers through unbiased selection.
  • Demonstrated high specificity of selected aptamers for senescent mouse cells in culture.
  • Identified fibronectin as the molecular target for two selected aptamers.
  • Observed increased aptamer staining in aged mouse tissues and decreased staining in specific cell-depleted models.

Conclusions:

  • Unbiased cell-based selection is an effective strategy for discovering novel, senescence-specific DNA reagents.
  • The developed DNA aptamers show significant potential as specific biomarkers for cellular senescence.
  • This approach offers a promising new avenue for the detection and study of senescence in biological systems.