Functional and structural profiling of circulation via genetically encoded modular fluorescent probes.

Area of Science:

• Physiology
• Biotechnology
• Animal Models

Background:

• Investigating fluid dynamics in animal models requires sustained labeling methods.
• Current techniques may lack the real-time monitoring capabilities needed for dynamic studies.

Purpose of the Study:

• To introduce a novel mouse line (Alb-mSc-ST) for sustained labeling of blood and interstitial fluids.
• To demonstrate the utility of this model for in vivo studies of fluid properties and vascular integrity.

Main Methods:

• Development of a genetically engineered mouse line (Alb-mSc-ST).
• Utilizing mScarlet fluorescent protein and SpyTag for fluid labeling.
• Application of SpyTag-SpyCatcher technology for monitoring and detection.

Main Results:

• Successful sustained labeling of both blood and interstitial fluid in the mouse model.
• Demonstrated capability to monitor circulating fluid properties using biosensors.
• Showcased the detection of blood-brain barrier disruption.

Conclusions:

• The Alb-mSc-ST mouse line is a valuable tool for in vivo investigation of fluid dynamics.
• This model facilitates the study of vascular structure, permeability, and organ microenvironments.
• Enables advanced research into physiological processes and disease mechanisms.