Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multiplexed Salivary miRNA Quantification for Predicting Severe COVID-19 Symptoms in Children Using Ligation-RPA Amplification Assay.

ACS sensors·2025

Same author

Solid-State Nanopore Sizing for cfDNA Sample Quality Control in Point-of-Need Sequencing.

ACS sensors·2025

Same author

Extracellular vesicle-based point-of-care testing for diagnosis and monitoring of Alzheimer's disease.

Microsystems & nanoengineering·2025

Same author

ViraLite: An Ultracompact HIV Viral Load Self-Testing System with Internal Quality Control.

medRxiv : the preprint server for health sciences·2025

Same author

Opportunities and challenges in implementing CRISPR-based point-of-care testing for Monkeypox detection.

BioTechniques·2025

Same author

Point-Of-Need One-Pot Multiplexed RT-LAMP Test For Detecting Three Common Respiratory Viruses In Saliva.

bioRxiv : the preprint server for biology·2025

Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026

Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026

Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026

Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026

Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026

Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026

See all related articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 16, 2025

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Solid-State Nanopore Sizing for cfDNA Sample Quality Control in Point-of-Need Sequencing.

Muhammad Asad Ullah Khalid¹, Md Ahasan Ahamed^1,2, Anthony J Politza³

¹Department of Intelligent System Engineering, Indiana University, Bloomington, Indiana 47408, United States.

Biorxiv : the Preprint Server for Biology

|April 1, 2025

Summary

This summary is machine-generated.

Portable nanopore sequencing needs on-site DNA quality control. This study introduces a solid-state nanopore device for label-free quantification of cell-free DNA (cfDNA), enabling accurate measurements at the point of need.

Keywords:

cell-free DNA nanopore plasma point-of-need quality control sequencing

More Related Videos

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Related Experiment Videos

Last Updated: May 16, 2025

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Area of Science:

Biotechnology
Genomics
Analytical Chemistry

Background:

DNA sequencing, including portable nanopore sequencing, is vital for disease diagnosis.
Current DNA sample quality control (QC) is lab-bound, limiting the utility of portable sequencers.
On-site QC is essential for personalized healthcare applications of portable sequencing.

Purpose of the Study:

To develop and validate a solid-state nanopore device for label-free quantification and qualification of cell-free DNA (cfDNA).
To enable accurate cfDNA assessment at the point of need, complementing portable sequencing technologies.

Main Methods:

Utilized a solid-state nanopore device for label-free cfDNA analysis.
Employed a 1 kbp double-stranded DNA internal marker for controlled quantification.
Investigated nanopore diameters (6-19 nm) and acquisition times for measurement consistency.
Compared nanopore assay results with capillary electrophoresis (CE) for plasma cfDNA samples.

Main Results:

Demonstrated controlled quantification of cfDNA using a DNA internal marker.
Achieved consistent measurements with nanopores (6-19 nm) with <15% CV.
Reduced measurement uncertainty to <10% CV by analyzing data from multiple nanopores over longer times.
Showed highly correlated results between the nanopore QC assay and CE for plasma cfDNA.

Conclusions:

The developed solid-state nanopore device offers a robust method for label-free cfDNA quantification and qualification.
This nanopore QC assay is suitable for point-of-need applications, enhancing the utility of portable DNA sequencing.
The technology shows significant potential for effective cfDNA assessment in personalized healthcare settings.