Complete genome sequences of two different broad bean stain virus isolates

Here, the complete genome sequences of two broad bean stain virus (BBSV; genus Comovirus) strains - Tunisia (RNA1, 5,965 nt; RNA2, 3,467 nt) and Syria (RNA1, 5,976 nt; RNA2, 3,449 nt) - were determined using next-generation sequencing (NGS) and rapid amplification of cDNA ends (RACE). These strains showed 79.2% and 71.6% nucleotide sequence identity to each other in RNA1 and RNA2, respectively. The predicted amino acid sequence identity was 95.2% in the proteinase-polymerase and 93.9% in the coat protein, indicating that these strains belong to the same species in the genus Comovirus. Phylogenetic analysis showed that both strains are closely related to red clover mottle virus (genus Comovirus). This is the first report of the complete genome sequences of two BBSV strains.