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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
Published on: December 1, 2016
Cepstrum-based interferometric microscopy with rolling-shutter cameras
Abstract:
Cepstrum-based interferometric microscopy (CIM) has risen as a groundbreaking digital holographic microscopy (DHM) technique that overcomes the conventional need of a clean/known reference beam for holographic recording. In CIM, two arbitrary complex object fields interfere each other to provide quantitative phase imaging (QPI) of both at once. Previous CIM implementations were validated using global-shutter (GS) cameras. However, rolling-shutter (RS) cameras provide several advantages, such as reduced price, high frame rate, low electronical noise, and small pixel size in spite of some drawbacks (flickering effect) that are overcome in this Letter. Hereby, we report on the validation of the CIM technique using rolling-shutter cameras including a novel, to the best of our knowledge, flickering correction approach. The performance of the approach is experimentally validated using calibrated phase objects and biological samples.
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