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Cepstrum-based interferometric microscopy with rolling-shutter cameras.

Ricardo Rubio-Oliver, Vicente Micó, Javier García

|April 1, 2025

Summary

This summary is machine-generated.

Cepstrum-based interferometric microscopy (CIM) now works with rolling-shutter cameras, overcoming previous limitations. This digital holographic microscopy technique offers cost-effective, high-speed quantitative phase imaging for various samples.

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Area of Science:

Optics and Photonics
Microscopy
Digital Holography

Background:

Conventional digital holographic microscopy (DHM) requires a clean reference beam for holographic recording.
Cepstrum-based interferometric microscopy (CIM) enables quantitative phase imaging (QPI) by interfering two object fields, eliminating the need for a reference beam.
Previous CIM implementations relied on global-shutter (GS) cameras, limiting advantages like cost and frame rate.

Purpose of the Study:

To validate the Cepstrum-based interferometric microscopy (CIM) technique using rolling-shutter (RS) cameras.
To introduce and validate a novel flickering correction approach for RS cameras in CIM.
To demonstrate the performance of RS-CIM with a flickering correction for calibrated phase objects and biological samples.

Main Methods:

Implementation of CIM using rolling-shutter cameras.
Development and application of a novel flickering correction algorithm tailored for RS-CIM.
Experimental validation using calibrated phase objects and biological specimens.

Main Results:

Successful validation of CIM with rolling-shutter cameras.
Demonstration of an effective flickering correction method for RS-CIM.
Quantitative phase imaging of calibrated and biological samples achieved using the improved RS-CIM.

Conclusions:

Rolling-shutter cameras are suitable for Cepstrum-based interferometric microscopy (CIM) with appropriate correction.
The novel flickering correction approach enhances the applicability of RS-CIM.
This advancement provides a more accessible and efficient method for quantitative phase imaging.