Cerebrospinal Fluid Phosphoproteome Characterization Using Titanium Dioxide Enrichment and High-Resolution LC-MS/MS

Cerebrospinal fluid (CSF) is a valuable source of biomarkers for neurological diseases due to its proximity to the central nervous system. Phosphorylation, a common post-translational modification, plays crucial roles in cellular signaling and is dysregulated in various neurological disorders.Analysis of the CSF phosphoproteome using titanium dioxide (TiO₂) enrichment coupled with high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique unveils a diverse array of phosphorylated proteins and their corresponding phosphorylation sites, shedding light on the intricate regulatory networks within CSF.The combination of TiO₂ enrichment and high-resolution LC-MS/MS offers a robust and sensitive approach to studying the phosphoproteome, providing valuable insights into cellular signaling dynamics and disease mechanisms.