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Analysis of Conformational Molecular Changes in Cerebrospinal Fluid Using Fourier Transform Infrared Spectroscopy in

Tanja Dučić1, Jan Christoph Koch2

  • 1CELLS-ALBA Synchrotron , Barcelona, Spain. tducic@cells.es.

Methods in Molecular Biology (Clifton, N.J.)
|April 1, 2025
PubMed

Keywords:
Bio-macromolecules, Neurodegenerative diseasesFTIR spectroscopyProtein secondary structure of CSF Conformational changes

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IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
The...