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Protective effects of hydrogen (H2) on boar sperm upon freeze-thaw process
1Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences; Shanghai Engineering Research Center of Breeding Pig, Shanghai, China.
Background:
Antioxidant is important to prevent ROS attack in boar sperm during the freezing-thawing process.
Objective:
To study whether hydrogen (H2) could improve the survival of boar sperm upon the freeze-thaw process.
Materials And Methods:
The effects of hydrogen in pre-diluent, freezing extender and thawing solution were investigated. Sperm viability, progressive motility, acrosomal integrity, mitochondrial activity, as well as the levels of malonaldehyde (MDA) and total antioxidant capacity (T-AOC) were measured.
Results:
Hydrogen in pre-diluent, freezing extender and thawing solution significantly improved sperm quality parameters (including sperm viability, progressive motility, acrosomal integrity and mitochondrial activity), increased total antioxidant capacity (T-AOC) and decreased malondialdehyde (MDA) of post-thawed boar sperm. The use of hydrogen in all three solutions improved the quality of post-thawed boar sperm.
Conclusion:
Hydrogen protects boar sperm against ROS-induced damages during cooling and the freezing-thawing process. Doi.org/10.54680/fr25310110512.
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