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Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
Published on: November 27, 2019
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Alternate Day Fasting Enhances Intestinal Epithelial Function During Aging by Regulating Mitochondrial Metabolism
Heng Quan1, Yao Lu2, Yingying Lin1
1Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, Beijing, China.
Aging Cell
|April 1, 2025
Summary
Alternate day fasting (ADF) rejuvenates aging intestinal stem cells (ISCs) by restoring their number and regenerative capacity. This dietary intervention combats age-related decline in intestinal function and barrier integrity.
Area of Science:
- Gastroenterology
- Aging Research
- Cellular Biology
Background:
- Aging leads to intestinal stem cell (ISC) dysfunction, increasing disease risk in the elderly.
- Senescent ISCs contribute to intestinal epithelium degeneration and impaired function.
- Dietary interventions are needed to target ISC aging and restore intestinal health.
Purpose of the Study:
- To investigate the impact of aging on intestinal stem cell function and intestinal health.
- To explore the potential of alternate day fasting (ADF) as an anti-aging dietary strategy for the small intestine.
Main Methods:
- Utilized a naturally aging mouse model (28 months old).
- Assessed intestinal barrier function and digestive absorption.
- Investigated mitochondrial function and metabolic capacity in intestinal stem cells.
- Examined the effects of fasting-refeeding, specifically alternate day fasting (ADF).
Main Results:
- Aging decreased ISC number and regenerative capacity, compromising small intestine barrier function and absorption.
- Mitochondrial degeneration in ISCs led to reduced energy supply and metabolic capacity.
- Alternate day fasting (ADF) restored ISC quantity and regenerative capabilities.
- ADF demonstrated a significant anti-aging effect on the small intestine.
Conclusions:
- Alternate day fasting (ADF) can ameliorate intestinal aging by rejuvenating intestinal stem cells.
- Fasting-refeeding influences ISC mitochondrial metabolism, offering a therapeutic avenue.
- This study provides a foundation for developing dietary interventions against age-related intestinal dysfunction.
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