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  1. Home
  3. Construction Of Saccharomyces Cerevisiae Platform Strain For The Biosynthesis Of Carotenoids And Apocarotenoids.
  1. Home
  3. Construction Of Saccharomyces Cerevisiae Platform Strain For The Biosynthesis Of Carotenoids And Apocarotenoids.

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Construction of Saccharomyces cerevisiae Platform Strain for the Biosynthesis of Carotenoids and Apocarotenoids.

Ping Lin1,2,3, Linpei Zhang2, Guocheng Du1,2,3

  • 1Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.

Journal of Agricultural and Food Chemistry
|April 1, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Microbial synthesis offers a sustainable alternative for producing carotenoids like beta-carotene (BC). This study engineered a yeast strain achieving record BC yields, enabling efficient production of valuable derivatives such as zeaxanthin and beta-ionone.

Keywords:
Saccharomyces cerevisiaebiosynthesissystematic metabolic engineeringβ-carotene chassis

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Area of Science:

  • Biotechnology
  • Metabolic Engineering
  • Synthetic Biology

Background:

  • Carotenoids and apocarotenoids are vital natural compounds.
  • Traditional plant extraction methods for these compounds are inefficient and polluting.
  • Microbial synthesis presents a sustainable and eco-friendly alternative.

Purpose of the Study:

  • To engineer a high-producing Saccharomyces cerevisiae platform strain for beta-carotene (BC) production.
  • To establish a robust platform for the microbial synthesis of BC and its derivatives.
  • To demonstrate the potential for producing downstream carotenoid derivatives.

Main Methods:

  • Systematic metabolic engineering of Saccharomyces cerevisiae.
  • Atmospheric and room temperature plasma mutagenesis for strain improvement.
  • Fed-batch fermentation in a 5-L bioreactor for BC production.
  • Introduction of key enzyme genes to engineer derivative-producing strains.

    • Main Results:

    • Achieved a record BC production of 2.09 g/L in Saccharomyces cerevisiae via fed-batch fermentation.
    • Engineered strains produced 39.09 mg/L of zeaxanthin and 31.87 mg/L of beta-ionone.
    • Demonstrated a significantly improved starting point for diverse BC derivative production.

    Conclusions:

    • The engineered Saccharomyces cerevisiae platform strain represents a significant advancement in microbial BC production.
    • This platform enables the sustainable and efficient synthesis of valuable carotenoid derivatives.
    • The study provides a foundation for further development in bio-based production of natural compounds.