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Related Concept Videos

Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

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Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Updated: May 16, 2025

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Engineering conjugative plasmids for inducible horizontal DNA transfer.

Tahani Jaafar1, Emily Carvalhais1, Arina Shrestha1

  • 1Department of Biochemistry, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 5C1, Canada.

Canadian Journal of Microbiology
|April 1, 2025
PubMed
Summary
This summary is machine-generated.

Researchers engineered inducible conjugative plasmids to combat antimicrobial resistance. This innovative approach enables controlled gene transfer for next-generation antimicrobials, enhancing safety and efficacy.

Keywords:
antifungalantimicrobialbiocontainmenthorizontal gene transferinducible conjugative machinery

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Area of Science:

  • Microbiology
  • Synthetic Biology
  • Biotechnology

Background:

  • Antimicrobial resistance (AMR) is a significant global health and food security threat, necessitating novel therapeutic strategies.
  • Existing chemical antimicrobials are becoming less effective due to widespread microbial resistance.
  • Conjugative plasmids offer a promising platform for developing next-generation antimicrobials by delivering toxic genetic payloads.

Purpose of the Study:

  • To develop inducible conjugative plasmids for controlled horizontal gene transfer.
  • To engineer biocontainment strategies for mitigating ecological risks associated with plasmid-based antimicrobials.
  • To identify bacterial genes crucial for stringent regulation of conjugative plasmid transfer.

Main Methods:

  • Development of arabinose-inducible conjugative plasmids.
  • Evaluation of 13 plasmids with single essential gene deletions.
  • Assessment of plasmid inducibility in cis and trans configurations.

Main Results:

  • Identification of trbC and trbF genes as key for stringent plasmid regulation.
  • Demonstrated significant inducibility (up to 5-log increase) of conjugation efficiency under induced conditions.
  • Confirmed inducibility in both cis and trans plasmid configurations.

Conclusions:

  • Engineered inducible conjugative plasmids provide a foundation for safer antimicrobial technologies.
  • Controlled plasmid transfer is achievable, addressing concerns of unintended gene spread.
  • Further optimization is needed to overcome challenges like reduced efficiency and promoter leakiness.