The preemptive control strategy for invasive plant seed banks triggering ecological threats through synchronized...

Invasive alien plants threaten global ecosystems by disrupting biodiversity and degrading ecological functions. Soil seed banks-the reservoirs of viable seeds in the soil-play a crucial role in the persistence and spread of plant populations. However, current control measures for invasive plants predominantly target above-ground vegetation, neglecting these underground seed reserves, thereby allowing invasive plants to re-establish their populations. Inducing synchronous germination to deplete seed banks offers a potential preemptive control strategy. This study hypothesizes that seeds of invasive plants secrete secondary metabolites to promote the synchronous germination of conspecific seeds. Focusing on Ambrosia trifida L., a globally harmful annual invasive plant, where the soil seed bank plays a crucial role in its continued impact. We used metabolomics to identify such metabolites and found that Angelicin significantly enhances germination rates by up to 116.9 % (P < 0.01). Field experiments conducted in the native habitat demonstrated that applying Angelicin at concentrations of 0.015 μg ml^-1 or higher depleted over 85 % of the seed bank (P < 0.01) without harming indigenous plant communities. These findings confirm the feasibility of depleting underground seed banks through induced germination. Integrating this strategy with traditional above-ground control methods can develop a comprehensive management system, offering a promising new approach for the widespread control of invasive plants.