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Updated: May 16, 2025
Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics
Published on: October 2, 2014
Preclinical Evaluation of HSP90 Noninvasive Imaging in Colorectal Cancer Diagnosis
Lenan Zou1, Qi Chang1, Chengwei Chen2
1State Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009, China.
Abstract:
Heat shock protein 90 (HSP90) is a powerful molecular chaperone responsible for the stability and integrity of various client proteins, including various transcription factors, kinases, and steroid hormone proteins, among others. Overexpression of HSP90 is closely related to cancer, immune diseases, and neurodegenerative diseases. However, there are few effective fluorescent probes for HSP90. Therefore, we synthesized a near-infrared fluorescent probe YQHSI-MPA-1 targeting HSP90. The probe was later structurally modified to improve the targeting specificity and accuracy in vivo as YQHSI-MPA-2. Furthermore, the application values of YQHSI-MPA-2 in situ CRC, AOM-DSS-induced CRC, and various metastasis models were evaluated. The results showed that YQHSI-MPA-2 had excellent specificity and high tumor contrast. In conclusion, YQHSI-MPA-2 is an ideal tool for tumor detection and surgical navigation.
