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Expression of miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters in peripheral blood mononuclear cells of ischemic

Tingting Feng1, Lizhen Wang1, Ruijiao Zhou1

  • 1Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Sichuan, China.

Scientific Reports
|April 1, 2025
PubMed

Insights

MicroRNA-1250-5p and microRNA-3065-5p show elevated levels in patients with ischemic stroke (IS). These microRNAs, along with microRNA-338-3p, correlate with disease severity and immune cell percentages in IS patients.

Area of Science:

  • Biochemistry
  • Genetics
  • Neurology

Background:

  • Ischemic stroke (IS) is a leading cause of long-term disability and mortality worldwide.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and are implicated in various diseases, including stroke.
  • Specific miRNA clusters may serve as potential biomarkers for IS diagnosis and prognosis.

Purpose of the Study:

  • To investigate the correlation between the miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters and ischemic stroke (IS).
  • To assess the relationship between these miRNA levels and clinical parameters, including stroke severity and immune cell profiles.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) were collected from 83 IS patients and 50 healthy controls.
  • Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to measure the expression levels of miRNA-338-3p, miRNA-1250-5p, and miRNA-3065-5p.
  • Statistical analyses were performed to correlate miRNA expression with IS diagnosis, disease progression (NIHSS scores), and immune cell percentages.

Main Results:

  • Expression of miRNA-1250-5p and miRNA-3065-5p was significantly higher in IS patients compared to controls.
  • No significant difference in miRNA-338-3p expression was found between groups.
  • miRNA-1250-5p levels fluctuated during the early stages of IS, while miRNA-3065-5p and miRNA-338-3p levels correlated with higher NIHSS and mRS scores.
  • The miRNA cluster positively correlated with neutrophil percentage and negatively with lymphocyte percentage.
  • All three miRNAs showed significant inter-correlation in IS patients.

Conclusions:

  • miRNA-1250-5p and miRNA-3065-5p may be potential biomarkers associated with ischemic stroke.
  • The expression patterns of these miRNAs suggest a role in the inflammatory response and disease progression in IS.
  • Further research is warranted to elucidate the precise mechanisms and clinical utility of these miRNA clusters in IS management.
Keywords:
Ischemic strokemiRNA clustersmiRNA-1250-5pmiRNA-3065-5pmiRNA-338-3p

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