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Published on: December 23, 2022
Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products
Hui-Juan Cheng1, Yue Liu1, Hua-Dong Li1,2
1Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative In-novation Center for Biomanufacturing Technology, East China University of Science and Technology, Shanghai 200237, China. pqma@ecust.edu.cn.
Abstract:
We combined catalytic hairpin assembly (CHA) with the Cas12a system for detecting melamine adulteration. This system involved two-step signal conversion and two-level amplification, boosting the sensor's versatility and sensitivity. The sensor showed excellent specificity and applicability for melamine detection in dairy products, and was broadened to viral nucleic acid detection.

