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Updated: May 17, 2025
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
A highly selective electrochemical aptasensor for Pb2+ based on molecular imprinting technology and tetrahedral DNA
Jiaqi Han1, Nianxin Zhu1, Jiahao Sha1
1Shanghai Engineering Research Center for Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Abstract:
A novel electrochemical biosensor for the detection of lead ions (Pb2+) with improved specificity and sensitivity was developed. The sensor design incorporated molecular imprinting technology, where chitosan was polymerized on the electrode surface to form a lead-specific cavity structure, thereby enhancing selectivity in complex sample matrices. Meanwhile, the tetrahedral DNA nanostructure was employed as the recognition probe to mitigate the entanglement issues commonly associated with single-stranded DNA, thus improving the sensitivity of the detection. The developed sensor exhibited a linear dynamic range from 0.050 to 2.000 μg/mL, with a limit of detection (LOD) of 0.0034 μg/mL. The aptasensor's efficacy was verified through the analysis of aquatic samples, demonstrating a high degree of reliability comparable to that of inductively coupled plasma mass spectrometry (ICP-MS).
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