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Three Rho of Plant (ROP)-GTPase regulatory proteins control ROP-mediated alkaloid biosynthesis in Catharanthus roseus
Anuj Sharma1,2, Sruthi Mohan1,2, Priyanka Gupta1,2
1Molecular Plant Biology and Biotechnology Lab, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru 560065, India.
Plant Physiology
|April 2, 2025
Summary
Specific Rho of plant (ROP)-GTPase regulatory proteins (RGRPs) regulate specialized metabolism in Madagascar periwinkle. These RGRPs interact with ROPs to control monoterpene indole alkaloid (MIA) biosynthesis.
Area of Science:
- Plant molecular biology
- Biochemistry
- Genetics
Background:
- Rho of plant (ROP)-GTPase regulatory proteins (RGRPs) are known regulators of plant morphogenesis, development, and immunity.
- The role of RGRPs in specialized plant metabolism, particularly in the biosynthesis of valuable compounds, remains largely unexplored.
Purpose of the Study:
- To investigate the function of RGRPs in regulating specialized metabolism in Madagascar periwinkle (Catharanthus roseus).
- To identify specific RGRPs and ROPs involved in monoterpene indole alkaloid (MIA) biosynthesis.
Main Methods:
- Yeast two-hybrid assays to identify RGRP-ROP interactions.
- Gene silencing and overexpression studies to assess the functional role of RGRPs in MIA production.
- Transcript expression analysis and protein localization studies.
Main Results:
- CrGEF1, CrGAP1, and CrGDI2 were identified as specific RGRPs interacting with CrROP3 and CrROP5.
- CrGEF1 positively regulates MIA biosynthesis, while CrGAP1 and CrGDI2 act as negative regulators.
- Functional domains within RGRPs are crucial for their regulatory activity in MIA biosynthesis.
Conclusions:
- Distinct RGRPs coordinate with specific ROPs to modulate transcription factors and fine-tune MIA biosynthesis in C. roseus.
- This study reveals a novel regulatory mechanism for specialized metabolism controlled by RGRP-ROP signaling pathways.
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