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Eight new C21-steroidal glycosides from the roots of Cynanchum auriculatum
Ya-Yu Wu1, Lan-Wen Gao2, Yu-Jun Tang1
1State Key Laboratory of Bioactive Molecules and Druggability Assessment, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 511443, PR China.
Abstract:
Eight new C21-steroidal glycosides (1-8) and fourteen known analogs (9-22) were isolated from the roots of Cynanchum auriculatum Royle ex Wight (Asclepiadaceae). The structures of the new compounds were elucidated on the basis of their IR, HR-ESI-MS, and NMR data. The cytotoxicities of compounds 1-22 were evaluated against two prominent human cancer cell lines, SW480 and MDA-MB-231, leading to preliminary structure-activity relationships (SARs). Notably, compounds 1, 5, 8, 10, and 22 exhibited significant cytotoxic activity against both cell lines, with IC50 values ranging from 8.76 ± 1.36 to 17.90 ± 1.77 μM for SW480 and from 10.97 ± 1.73 to 12.07 ± 1.13 μM for MDA-MB-231.
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