Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
RNA Splicing01:32

RNA Splicing

55.8K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.8K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K
MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

857
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
857
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

22.1K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward the precision use of Chinese PARP inhibitors in ovarian cancer: clinical progress, resistance mechanisms, and future perspectives.

Cancer biology & medicine·2026
Same author

Inference of upstream-mutation and metabolomic-signature causality identifies prognostic biomarkers and therapeutic targets in pancreatic cancer.

Nature communications·2026
Same author

c-Myc/GRPEL1 maintains fatty acid synthesis via FASN to support PDAC cell proliferation.

Cell death & disease·2026
Same author

Identification of PIWI-interacting RNAs based models for lung adenocarcinoma early detection: a multicenter cohort study.

Molecular biomedicine·2025
Same author

The integral membrane protein smim4 modulates redox balance via malate compartmentalization in pancreatic cancer.

Nature communications·2025
Same author

Peripheral immune and inflammatory markers as predictors of neoadjuvant immunotherapy response in head and neck squamous cell carcinoma.

Annals of medicine·2025

Related Experiment Video

Updated: May 20, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

8.9K

ALKBH5-mediated m6A regulates the alternative splicing events of SRSF10 in ovarian cancer.

Kexin Li1, Yuqing Pei2,3,4, Xin Dong1

  • 1Department of Clinical Laboratory, State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Cancer Gene Therapy
|April 2, 2025
PubMed
Summary

N6-methyladenosine (m6A) demethylase ALKBH5 promotes ovarian cancer by affecting RNA splicing. Targeting ALKBH5-mediated m6A modification offers a new therapeutic strategy for ovarian cancer (OC).

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

12.9K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

4.8K

Related Experiment Videos

Last Updated: May 20, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

8.9K
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

12.9K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

4.8K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Oncology

Background:

  • N6-methyladenosine (m6A) methylation plays a role in ovarian cancer tumorigenesis.
  • The precise mechanism by which m6A demethylase ALKBH5 influences RNA splicing in ovarian cancer remains unclear.

Purpose of the Study:

  • To investigate the role of ALKBH5 in ovarian cancer.
  • To elucidate the mechanism of ALKBH5-mediated m6A modification in RNA splicing and its impact on ovarian cancer progression.

Main Methods:

  • Examined ALKBH5 protein expression and m6A levels using immunohistochemistry.
  • Utilized siRNA and CRISPR/Cas9 knockout to deplete ALKBH5 in ovarian cancer cells (A2780).
  • Performed MeRIP-seq and RNA-seq to analyze ALKBH5-regulated m6A modifications and their effects on RNA splicing.

Main Results:

  • Elevated ALKBH5 expression and reduced m6A levels were observed in ovarian cancer patients.
  • ALKBH5 depletion inhibited ovarian cancer cell viability, proliferation, and migration.
  • ALKBH5 regulates RNA splicing, impacting alternative splicing of the SRSF10 gene, specifically affecting exon 5 retention and SRSF10-211 transcript expression.

Conclusions:

  • ALKBH5-mediated m6A modification significantly impacts RNA splicing in ovarian cancer.
  • The findings suggest ALKBH5 as a potential therapeutic target for ovarian cancer.
  • This study provides novel insights into m6A modification mechanisms for ovarian cancer treatment.