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Related Experiment Video

Updated: May 17, 2025

Methodology for Accurate Detection of Mitochondrial DNA Methylation
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Method for Detecting Mitochondrial ATP by Hybridization Chain Reaction.

Lei Luo1, Xiaohai Yang2, Kemin Wang3

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China.

Methods in Molecular Biology (Clifton, N.J.)
|April 2, 2025
PubMed
Summary

This study details a new method for imaging cellular energy production by tracking adenosine triphosphate (ATP) within mitochondria. The protocol uses hybridization chain reaction (HCR) for reliable detection in living cells.

Keywords:
Adenosine triphosphate (ATP)Hybridization chain reactionImagingMitochondria

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Imaging

Background:

  • Adenosine triphosphate (ATP) is crucial for cellular energy transduction and signaling.
  • Accurate detection of mitochondrial ATP is essential for understanding cellular metabolism.
  • Existing probes face challenges in noninvasive cell entry, mitochondrial targeting, and reliable ATP response.

Purpose of the Study:

  • To develop a robust protocol for imaging mitochondrial ATP in living cells.
  • To leverage hybridization chain reaction (HCR) for enhanced ATP detection sensitivity.
  • To provide a reliable method for studying cellular energy dynamics.

Main Methods:

  • Utilizing a detailed protocol for live-cell imaging.
  • Employing hybridization chain reaction (HCR) for signal amplification.
  • Focusing on noninvasive cellular entry and specific mitochondrial targeting of probes.

Main Results:

  • Successful imaging of mitochondrial ATP in living cells.
  • Demonstration of reliable ATP detection using the HCR-based approach.
  • Validation of the protocol's efficacy for cellular energy studies.

Conclusions:

  • The developed HCR-based protocol enables effective noninvasive imaging of mitochondrial ATP.
  • This method offers a reliable tool for investigating cellular energy metabolism and signaling.
  • The protocol advances mitochondrial ATP detection in live-cell research.