Proteomic insights into lymph node metastasis in breast cancer subtypes: Key biomarkers and pathways
- 1Department of Medical Biology, Medical School, Kocaeli University, Kocaeli 41001, Turkiye.
- 2Department of General Surgery, Medical School, Kocaeli University, Kocaeli 41001, Turkiye.
- 0Department of Medical Biology, Medical School, Kocaeli University, Kocaeli 41001, Turkiye.
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View abstract on PubMed
Summary
This summary is machine-generated.This study identifies key proteins like CAPZB and CORO1A involved in breast cancer metastasis and patient outcomes. These findings offer potential new therapeutic targets for personalized breast cancer treatment.
Area Of Science
- Oncology
- Molecular Biology
- Proteomics
Background
- Breast cancer (BC) remains a major global health challenge for women, largely due to its heterogeneity and propensity for metastasis.
- Understanding the molecular drivers of BC metastasis is crucial for improving patient survival rates.
Purpose Of The Study
- To identify differentially expressed proteins in breast cancer subtypes associated with metastasis.
- To explore the prognostic and diagnostic relevance of these identified proteins.
- To elucidate molecular pathways driving breast cancer invasion and metastasis.
Main Methods
- Proteomic analysis of breast cancer, healthy lymph node, and metastatic lymph node tissues using 2D gel electrophoresis and MALDI-TOF/TOF mass spectrometry.
- Bioinformatic analysis including protein-protein interaction networks and pathway enrichment (STRING).
- Prognostic evaluation using Kaplan-Meier analysis (KM plotter) and diagnostic assessment via Receiver Operating Characteristic (ROC) curves (TCGA, GTEx data).
Main Results
- Proteins involved in cytoskeletal regulation, immune modulation, and oxidative stress were differentially expressed across BC subtypes.
- Key proteins such as TUBA1C, CCT6A, Vimentin, CAPZB, ENO1, GSTO1, CORO1A, and LAP3 were identified as significant in metastatic behavior.
- CAPZB and CORO1A showed prognostic significance, GSTO1 correlated with improved metastasis-free survival in HER2-overexpressing (OE) BC, and CAPZB demonstrated strong diagnostic potential.
Conclusions
- The study provides a foundation for understanding the molecular mechanisms of metastasis in diverse breast cancer subtypes.
- Identified proteins may serve as novel therapeutic targets for developing targeted interventions against cancer invasion and metastasis.
- Further clinical validation in larger patient cohorts is necessary to confirm the utility of these findings.
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