Preparation and characterization of thermosensitive phase-transition hydrogel based on decanoic acid-modified...

Hydrogels with good biocompatibility, suitable physicochemical properties, and effective wound healing promotion are currently recognized as ideal candidates for wound dressings. This study introduced an innovative thermosensitive phase-transition hydrogel (CSDA-MC-HG) for skin wound repair, prepared using decanoic acid-modified chitosan (CSDA) and methyl cellulose (MC). The enhanced hydrophobic interaction with increasing temperature was the primary mechanism behind the thermosensitive phase-transition property of CSDA-MC-HG. Rheological measurement confirmed that CSDA-MC-HG possessed adequate spreadability and adaptability, allowing it to conform well to irregular shaped wounds and be easily applied and replaced. The other characterization findings indicated that CSDA-MC-HG possessed ideal interconnected porous structure, along with superior swelling capacity, water retention ability, and water vapor permeability necessary for an optimal wound dressing. Biocompatibility experiments indicated that CSDA-MC-HG exhibited satisfactory blood compatibility and cell compatibility, supporting the proliferation and migration of L929 cells. Furthermore, the hydrogel's potential as a wound dressing was tested on SD rats with full-thickness skin wounds. The results indicated that CSDA-MC-HG effectively promoted wound healing by enhancing fibroblast proliferation, accelerating the formation of new blood vessels and skin appendages, and facilitating collagen deposition. The findings presented suggested that CSDA-MC-HG held significant potential for application as a wound healing dressing.