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Updated: May 2, 2026

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
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Optimization and Application of the Purified Cell-Free System

Chao Liao1,2, Hengrun Li1,2, Haotian Zheng1,2

  • 1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Chembiochem : a European Journal of Chemical Biology
|April 4, 2025
PubMed

Keywords:
PURE systemnon‐canonical amino acidone‐pot preparationprototyping researchsynthetic cell

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