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Updated: May 16, 2025

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
Published on: December 11, 2012
Atypical pathogenesis of DsCPV-1 in candidate for mass production Manduca sexta (Lepidoptera: Sphingidae)
Anna O Subbotina1,2, Vyacheslav V Martemyanov1, Irina A Belousova1
1Institute of Systematics and Ecology of Animals SB RAS, Novosibirsk, Russia.
Abstract:
Recently obtained cypovirus strain DsCPV-1 shows potential as a biological insecticide, with its alternative host Manduca sexta (L.) being a promising producer. We confirmed DsCPV-1 replication in M. sexta by quantitative PCR, validating DsCPV-1's suitability as a biological producer. At the terminal infection stage, we revealed many nonoccluded DsCPV-1 virions by transmission electron microscopy, indicating virus replication with reduced or without polyhedron formation in this alternative host.

