Nucleolar changes in goldfish retinal ganglion cells in response to an optic nerve lesion are not perturbed by a second lesion

The reaction of the goldfish retinal ganglion cells to optic nerve crush, as indicated by an increase in nucleolar incidence 4 to 5 days after this lesion, was not significantly affected if a second nerve crush was made within 3 days after the first. This appears to rule out axon sprouting as the initiating event for the cell body reaction to axotomy.