Novel poly-deep eutectic solvent-functionalized magnetic graphene oxide nanomaterials for high-performance...

Area of Science:

• Materials Science
• Biochemistry
• Analytical Chemistry

Background:

• Developing efficient methods for selective protein extraction is crucial in biochemical analysis.
• Magnetic graphene oxide composites offer unique properties for solid-phase extraction.
• Deep eutectic solvents (DES) present tunable characteristics for material functionalization.

Purpose of the Study:

• To design and synthesize novel polymethacrylic acid-based deep eutectic solvent (DES)-functionalized magnetic graphene oxide composites.
• To evaluate the efficiency and selectivity of these composites for the solid-phase extraction of trypsin (Tryp).
• To investigate the reusability and application of the optimized extractant in a complex biological matrix.

Main Methods:

• Radical polymerization was employed to synthesize three polymethacrylic acid-based DES-functionalized magnetic graphene oxide composites.
• Solid-phase extraction (SPE) was utilized to isolate and purify trypsin.
• Characterization of the material properties and extraction performance was conducted under optimized conditions.
• Selectivity was assessed against seven different biomacromolecules.

Main Results:

• The MGO@PDES2 extractant, utilizing a specific DES composition (tetraethylammonium chloride and methylpropionic acid, 1:2 molar ratio), demonstrated the highest extraction efficiency for trypsin.
• The optimized MGO@PDES2 achieved a high extraction capacity of 708.85 mg g⁻¹ for trypsin, driven by hydrogen-bonding and electrostatic interactions.
• Outstanding selectivity for trypsin was observed among seven tested biomacromolecules, with excellent reusability over multiple cycles.
• Successful application of the method for trypsin extraction from bovine pancreas crude extract confirmed its practical viability.

Conclusions:

• MGO@PDES2 is a highly effective and selective magnetic extractant for trypsin.
• The developed composite material offers a promising and reusable solution for protein extraction and separation.
• This approach provides novel insights for advancing protein purification techniques in biological and analytical chemistry.