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Published on: August 29, 2012
Immortalized porcine mesangial cell line competent for the cultivation of Japanese encephalitis virus
Shucheng Zong1, Xinze Liu2, Wen Pan2
1Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
Importance:
The Japanese encephalitis virus (JEV) is a massive threat to the pig-raising industry and attenuated-live vaccines are used widely to prevent JEV infections. Primary hamster kidney cells and Vero cells are mainly used to propagate JEV-attenuated vaccines.
Objective:
This study aimed to construct porcine mesangial cells (PMCs) for JEV cultivation and study its characteristics.
Methods:
An immunofluorescence assay was used to analyze the homogeneity of PMCs. Karyotypic analysis and flow cytometry were used to detect the cell chromosome numbers and cell cycle, respectively. TCID₅₀ was used to measure the viral titers.
Results:
The prepared PMCs were uniform in shape and without variation in chromosome numbers. The PMCs exhibit strong division and proliferation ability, and the JEV could replicate efficiently, having a similar cytopathic effect to that in Vero cells. The viral titer results showed that the JEV grows faster in PMCs than in Vero cells.
Conclusions And Relevance:
This study generated an immortalized PMC cell line suitable for cultivating the JEV, which has significance for producing pig JEV-attenuated vaccines.
