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Related Experiment Video

Updated: May 16, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Protocol for label-free quantitative lysine lactylproteome profiling.

Yu Ran¹, Qiqing Yang², Lianqi Zhou²

¹Department of Radiation Oncology and the State Key Laboratory of Transvascular Implantation Devices, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China; The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, The First Affiliated Hospital, Jiangxi Medical College Nanchang University, Nanchang, China.

|April 4, 2025

Summary

This summary is machine-generated.

This study introduces a new protocol for mapping lactylation, a key protein modification. This method enables global profiling and quantification of lactylated proteins using mass spectrometry.

Keywords:

Mass spectrometry Protein expression and purification Proteomics

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Last Updated: May 16, 2025

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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

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Published on: April 19, 2019

Area of Science:

Biochemistry
Proteomics
Cellular Biology

Background:

L-lactate is crucial for cellular signaling and metabolic homeostasis.
Lactylation, a post-translational modification (PTM) from L-lactate, impacts protein function and cellular processes.

Purpose of the Study:

To present a comprehensive protocol for global lactylation proteome profiling.
To enable label-free quantification of lactylated proteins.

Main Methods:

Detailed steps for cell preparation and protein extraction.
Procedures for protein digestion, peptide desalting, and lactylpeptide enrichment.
Parameters for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.

Main Results:

The protocol facilitates global profiling of the lactylation proteome.
Enables label-free quantification of lactylated peptides.
Provides a reproducible method for studying lactylation.

Conclusions:

This protocol offers a robust approach for investigating the role of lactylation in biological systems.
It aids in understanding the functional significance of lactylation in cellular signaling and metabolism.